QUANTITATION OF CARBOHYDRATE DEFICIENT TRANSFERRIN IN HIGH ALCOHOL CONSUMPTION BY HPLC

摘要

A method for separation and quantitation of carbohydrate deficient transferrin (CDT) in order to decide a person's alcohol consumption during the most recent time. Lipoproteins are precipitated in blood serum, saturated with iron, and then the sample is centrifuged after a short storage period. The supernatant of the centrifuged mixture is then diluted with water. Subsequently the mixture is injected to an ion exchange column, when a saltgradient is used for separation of the isoforms of transferrin. A chromatogram is developed by means of HPLC and a filter for absorbance at 460 nm. Should the chromatographic profile show increased values of CDT (*, **), more than 0.8 % of total transferrin, it is a highly specific marker for heavy alcohol consumption.