Method for mass production of recombinant proteins by a novel expression system consisting of a promoter of the autografpa california nuclear polyhedral disease virus GP64 and a fusion promoter of a polyhedral promoter

摘要

The present invention provides a novel fusion promoter in which a polyhedrin promoter of Autographa Californica nuclear polyhedrosis virus (hereinafter abbreviated as 'AcNPV') is fused with a promoter of gp64, an envelope glycoprotein, A baculovirus expression vector comprising a signal sequence of gp64 and a foreign gene sequence, and a method for producing and expressing a large amount of foreign protein in insect cells using the same, specifically, a polymorphic promoter of AcNPV Under the control of the fusion promoter fused with the hydrin promoter and the early and late promoters of gp64, a baculovirus expression vector capable of expressing a foreign protein fused with the signal sequence of gp64 was constructed and homologous with the wild type baculovirus. Recombination) obtained three recombinant insect baculoviruses It was infected to express a foreign protein in large quantities in the form of active-form, relates to a method of making.;By using the baculovirus expression vector of the present invention, useful foreign proteins can be easily isolated and purified from cell culture medium in a form that is active in the human body. As expression is maintained at a high level, it can be produced in large quantities.