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METHOD FOR MASS-PRODUCING RECOMBINANT PROTEIN BY NOVEL EXPRESSION SYSTEM COMPRISING FUSION EXPRESSION VECTOR OF POLYHYDRIN PROMOTOR OF AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS ( ACNPV) AND PROMOTOR OF GP64
METHOD FOR MASS-PRODUCING RECOMBINANT PROTEIN BY NOVEL EXPRESSION SYSTEM COMPRISING FUSION EXPRESSION VECTOR OF POLYHYDRIN PROMOTOR OF AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS ( ACNPV) AND PROMOTOR OF GP64
PURPOSE: Provided are Baculovirus expression vector comprising a fusion promotor wherein a polyhydrin promotor of Autographa Californica nuclear polyhedrosis virus (AcNPV) and a promotor of envelope protein glycoprotein gp64 are fused together and signal sequence of gp64 and foreign genetic sequence, and a method for mass-producing a recombinant protein. The Baculovirus vector enables separation and purification of a useful foreign protein as an active form from the cell culture and to increase the expression quantity. CONSTITUTION: The Baculovirus expression vector, pPGP404, includes a fusion promotor wherein polyhydrin promotor including nucleotide sequence of SEQ ID NO:9 originated from Autographa Californica nuclear polyhedrosis virus (AcNPV) and a promotor of envelope protein glycoprotein gp64 including 1-292 bases of nucleotide sequence of SEQ ID NO:10 are fused together. E.coli transformant, TOP10F'/pPGP404(KFCC-11031) is prepared by transforming E.coli TOP10F' strain with the expression vector pPGP404.
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机译:目的:提供杆状病毒表达载体,其包含融合启动子,其中Autographa Californica核多角体病毒(AcNPV)的多元醇启动子和包膜蛋白糖蛋白gp64的启动子融合在一起,并且gp64的信号序列和外来遗传序列以及其制备方法-产生重组蛋白。杆状病毒载体能够从细胞培养物中分离和纯化有用的外源蛋白作为活性形式,并增加表达量。组成:杆状病毒表达载体pPGP404,包括一个融合启动子,其中包括SEQ ID NO:9核苷酸序列的多羟甲基启动子,该核苷酸来源于Autographa Californica核多角体病毒(AcNPV),而包膜蛋白糖蛋白gp64的启动子包括1-292个碱基将SEQ ID NO:10的序列融合在一起。通过用表达载体pPGP404转化大肠杆菌TOP10F'菌株来制备大肠杆菌转化体TOP10F'/ pPGP404(KFCC-11031)。
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