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Recombinant interleukin-1 β converting enzyme gene, expression vector comprising the same, and method for producing active interleukin-1 β converting enzyme in E. coli using the same
Recombinant interleukin-1 β converting enzyme gene, expression vector comprising the same, and method for producing active interleukin-1 β converting enzyme in E. coli using the same
The present invention relates to a recombinant gene of Interleukin-1 β Converting Enzyme (hereinafter, abbreviated as 'ICE'), a protein involved in apoptosis of cells, an expression vector including the same, and the expression The present invention relates to a method for producing a recombinant ICE protein using a vector. Specifically, the recombinant genes of p10 and p20 subunits of ICE, which are auto-processed upon activation, are expressed by a bistronic method. The present invention relates to a method for producing an active ICE in Escherichia coli using the expression vector and the expression vector. According to the present invention, the expression of p10 and p20 monomers is simultaneously expressed. It can be manufactured in large quantities.
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