Differentiation detection method of anti-tubercle bacillus and anti-tubercle bacillus with RPOB gene

摘要

The present invention relates to a gene encoding an RNA polymerase beta subunitrPoB) Were used as targets.Mycobacterium tuberculosisA primer pair for duplex-polymerase chain reaction (duplex-PCR) that can differentiate between mycobacterium tuberculosis complex and Mycobacteria other than Tubercle bacilli (MOTT) The present invention relates to a method for diagnosing tuberculosis and MOTT infection as a detection method. In the present invention,rPoBWe have developed a pair of primers for double PCR that can be detected easily and effectively by discriminating between Mycobacterium tuberculosis and MOTT. PCR is carried out simultaneously using the two specific primer pairs to determine the type of template DNA present in the specimen,rPoBThe DNA was amplified in a single band of different size, and thus it was possible to confirm the infection according to its size. Therefore, according to the double PCR method using the primer pair of the present invention, a single copy exists in all mycobacteria,rPoBBy targeting only one gene, it is possible to differentiate and detect the Mycobacterium tuberculosis group and MOTT more easily and specifically than any conventional method.