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Method of detecting influenza bacillus, primer set for detection of influenza bacillus and kit for detection of influenza bacillus
Method of detecting influenza bacillus, primer set for detection of influenza bacillus and kit for detection of influenza bacillus
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机译:检测流感杆菌的方法,用于检测流感杆菌的引物组和用于检测流感杆菌的试剂盒
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摘要
The present invention provides a method of detecting Haemophilus influenzae, which enables accurate and rapid detection of H. influenzae, a primer set for detecting H. influenzae, and a kit for detecting H. influenzae. ;Nucleic acid amplification is carried out using the DNA of H. influenzae as a template, and also using the LAMP primers as shown in SEQ ID NOS: 1 to 5 given as examples of the present invention. Thus, the presence or absence of the amplified product is detected. When primers having sequences that are complementary to the sequences as shown in SEQ ID NOS: 1 to 5 are used, such primers are excellent in terms of detection sensitivity and promptness of detection, as well as specificity. In addition, as another example of the present invention, nucleic acid amplification is carried out using LAMP primers as shown in SEQ ID NOS: 43 to 47, and the presence or absence of the amplified product is detected. Thereby, H. influenzae Type b can be distinguished from other capsular serotype and non-encapsulated type H. influenzae, and it can be detected rapidly, simply, and accurately.
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机译:本发明提供了一种检测流感嗜血杆菌 I>的方法,该方法能够准确且快速地检测 H。流感 I>,用于检测 H的引物。流感 I>和用于检测 H的试剂盒。流感。 I>;使用 H的DNA进行核酸扩增。流感 i>作为模板,并且也使用作为本发明实例给出的SEQ ID NOS:1-5所示的LAMP引物。因此,检测到扩增产物的存在或不存在。当使用具有与SEQ ID NO:1至5所示的序列互补的序列的引物时,这种引物在检测灵敏度和检测迅速性以及特异性方面是优异的。另外,作为本发明的另一个例子,使用SEQ ID NO:43至47所示的LAMP引物进行核酸扩增,并且检测扩增产物的存在或不存在。从而, H。 b型流感 I>可以与其他荚膜血清型和非荚膜 H型区分开。流感 I>,并且可以快速,简单和准确地对其进行检测。
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