METHOD FOR PREPARING ENZYME VARIANTS HAVING IMPROVED TRAITS, COMPRISING HIGH SPEED SELECTION METHOD

摘要

PURPOSE: A preparation method of enzyme variants having improved traits is provided which comprises a high speed selection method therefore it is able to perform a speedy search step and a selection step of enzyme variants. CONSTITUTION: A method for preparation of enzyme variants comprises the following steps: (a) mutagenesis of enzyme gene; (b) insertion of the mutated enzyme gene into an expression vector having a surface-expression protein gene and then construction of enzyme gene variants library to be introduced into a microbe host; (c) expression of the enzyme gene variants library as the surface of microbe host; and (d) search and selection of microbe hosts exhibiting improved multiplication velocity or enzymatic activity.