METHOD FOR PREPARING ENZYME VARIANTS HAVING IMPROVED TRAITS, COMPRISING HIGH SPEED SELECTION METHOD

摘要

The present invention relates to a selection method for enzyme variants with improved traits, including high-speed seonbyeolbeop, and more particularly mutagenesis step of the gene; Construction phase of the combined mutations induced gene expression vector containing the gene expression of surface proteins, then gene variant libraries to be introduced into the host microorganism; Step constructed expression of the enzyme to the surface of the microbial host a gene variant libraries; And showing an increased growth rate or enzymatic activity related to a screening method of the enzyme variant containing the microorganism host navigation and selection steps, it is possible to quickly perform a search and selection phase of the enzyme variant effect that facilitates the entire process a.