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EXPRESSION VECTORS CONTAINING HUMAN GLUTAMINE SYNTHASE GENE AND PROCESS FOR PRODUCING ERYTHROPOIETIN FROM ANIMAL CELL USING THE SAME
EXPRESSION VECTORS CONTAINING HUMAN GLUTAMINE SYNTHASE GENE AND PROCESS FOR PRODUCING ERYTHROPOIETIN FROM ANIMAL CELL USING THE SAME
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机译:含有人谷氨酰胺合成酶基因的表达载体和使用该载体从动物细胞生产促红细胞生成素的方法
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PURPOSE: Provided are an expression vectors containing human glutamine synthase gene and a process for producing erythropoietin from an animal cell using the same vector, thereby producing the erythropoietin having improved activity in a higher yield. CONSTITUTION: The expression vector capable of mass-expressing a foreign gene from an animal cell is produced by inserting a human glutamine synthase gene connected to the downstream of a SV40 promoter, wherein 128 to 270 bases are removed, into an expression vector pcDNA, in which the expression vector pcDNA-gs has a restriction enzyme map of figure 3. A transformant E. coli/pcDNA-gs (KCTC 8860 P) is produced by transformation of E. coli with the expression vector pcDNA-gs. The expression vector pcDNA-EPO-dE1-gs is capable of producing erythropoietin in a higher yield. A transformant E. coli/pcDNA-EPO-dE1-gs is produced by transformation of E. coli with the expression vector pcDNA-EPO-dE1-gs. The erythropoietin is produced by fermenting the transformants in a methionine sulfoximine-increased medium to select the cell lines capable of producing erythropoietin in a higher yield and fermenting the cell lines.
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