An expression vector containing human glutamine synthase gene and a producing method of erythropoietin from animal cell using the same

摘要

PURPOSE: Provided are an expression vectors containing human glutamine synthase gene and a process for producing erythropoietin from an animal cell using the same vector, thereby producing the erythropoietin having improved activity in a higher yield. CONSTITUTION: The expression vector capable of mass-expressing a foreign gene from an animal cell is produced by inserting a human glutamine synthase gene connected to the downstream of a SV40 promoter, wherein 128 to 270 bases are removed, into an expression vector pcDNA, in which the expression vector pcDNA-gs has a restriction enzyme map of figure 3. A transformant E. coli/pcDNA-gs (KCTC 8860 P) is produced by transformation of E. coli with the expression vector pcDNA-gs. The expression vector pcDNA-EPO-dE1-gs is capable of producing erythropoietin in a higher yield. A transformant E. coli/pcDNA-EPO-dE1-gs is produced by transformation of E. coli with the expression vector pcDNA-EPO-dE1-gs. The erythropoietin is produced by fermenting the transformants in a methionine sulfoximine-increased medium to select the cell lines capable of producing erythropoietin in a higher yield and fermenting the cell lines.