Recombinant Drosophila deoxynucleotide kinase useful for preparing nucleoside monophosphates by phosphorylating nucleosides

摘要

A recombinant kinase (I) obtainable from insect cells, which is stable during nucleoside monophosphate synthesis without the addition of stabilizing sulfhydryl reagent or stabilizing proteins and accepts all four natural deoxynucleotides, is new. Independent claims are also included for the following: (1) a DNA sequence (II) encoding (I); (2) a vector (III) containing (II) and a promoter; (3) a host cell (IV) transformed with (III); (4) producing (I); and (5) preparing a nucleoside monophosphate by using (I) to phosphorylate a nucleoside.