Inorganic carrier containing bound silane coupling agent having carboxylic-ester group for immobilizing lipase

摘要

A carrier for immobilizing lipase is prepared by bonding a silane coupling agent having a carboxylic-ester group to an inorganic carrier. Preferred silane coupling agents are &ggr;-methacryl oxypropyltrimethoxy silane, &ggr;-acetoxypropyltrimethoxy silane or . gamma.-acryloxypropyltrimethoxy silane. The inorganic carrier can be kaolinite, porous glass, bentonite, silica gel, alumina, silica, silica- alumina hydroxy apatite or calcium phosphate gel. Lipase is immobilized by mixing lipase with the inorganic carrier containing the bound silane coupling agent, filtering the resultant mixture and drying the filtered mixture. Functional groups of the lipase are bonded to the carboxylic- ester group of the silane coupling agent.