Manner and kitsuto null in order to measure thyrosin phosphorylation and dephosphorization activity

摘要

A safe and sensitive method is provided for determining the tyrosine-phosphorylating or phosphotyrosine-dephosphorylating activity of a material, such as a human tissue sample, by reacting the sample with a polypeptide which contains (phospho)tyrosine residues and assaying the phosphorylated polypeptide by using an antibody to a polypeptide containing phosphotyrosine. The method can conveniently be performed using a diagnosis kit containing the polypeptide and the antibody. The method and the kit are useful for example for diagnosing tumours.