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Assay of nucleotides in solution using fluorescence intensity quenching effect
Assay of nucleotides in solution using fluorescence intensity quenching effect
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机译:利用荧光强度猝灭作用测定溶液中的核苷酸
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摘要
The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using laser induced fluorescence of antisense probes, including PNA probes. Fluorescent intensity of the resulting medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence. The method is particularly advantageous in not requiring separation of unhybridized probes and hybridization complexes prior to detection. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.
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