Capillary electrophoresis molecular weight separation of biomolecules using a polymer-containing solution

摘要

A capillary tube with a charged inner surface for use in capillary electrophoresis and methods. The tube is filled with an electrolyte solution containing 0.05-30 % weight/weight of a hydrophilic polymer which is characterized by (A) a molecular weight of 20-5,000 kilodaltons, and (B) a charge between 0.01 and 1.0 % as measured by the molar percent of charged monomer subunits to the total polymer subunits, where said charged monomer subunits have the charge opposite to the wall charge at a selected electrophoresis pH.