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METHOD FOR DETECTING VIROID AND CAPTURE PROBE USED FOR DETECTION OF VIROID

机译:检测病毒的方法和用于检测病毒的捕获探针

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting viroid, capable of stably detecting viroid in a high sensitivity by a simple operation without spending time and effort and provide capture probe used for detection of viroid. ;SOLUTION: This method for detecting viroid comprises a step for extracting RNA from a plant which becomes a specimen, a step for recovering only viroid RNA from the extracted RNA by using capture probe with a base sequence having complementarity with base sequence site which is common to well-known plural viroid RNAs and a step for amplifying gene by gene amplification method after reversely transferring the recovered viroid RNA to cDNA.;COPYRIGHT: (C)2001,JPO
机译:解决的问题:提供一种检测类病毒的方法,该方法能够通过简单的操作以高灵敏度稳定地检测类病毒,而无需花费时间和精力,并且提供了用于检测类病毒的捕获探针。 ;解决方案:这种检测类病毒的方法包括从成为标本的植物中提取RNA的步骤,通过使用具有与碱基序列位点互补的碱基序列的捕获探针仅从提取的RNA中回收类病毒RNA的步骤。回收著名的类病毒RNA,并将回收的类病毒RNA反向转移至cDNA后,通过基因扩增方法扩增基因的步骤。;版权所有:(C)2001,日本特许厅

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