How to isolate and map in vivo chromosomal regions that bind or interact function on the chromosome or between chromosomes

摘要

(57) A method of identifying a region of a chromosome that interacts functionally with chromosome or between chromosomes, [Abstract] The present invention comprises the following steps: directly in the target sequence present in the chromosome of a) at least one recombining chain recipient vector, but at that time, the above-mentioned recipient vector includes at least a recipient array consisting of a nucleic acid sequence complementary to recombine the above-mentioned target sequence and tail consisting of a heterologous nucleic acid sequence; b) PCR and on method comprising identifying at least one region of the chromosome which are capable of detecting the tag label and / or on the target sequence or the recipient vector or by means to recombine the recipient vector.