EXTREMELY RAPID TRANSFORMATION OF MONOCOTY LEDON

摘要

PROBLEM TO BE SOLVED: To provide a method for more efficiently and more rapidly transforming a monocot such as Oryza sativa, Triticum sativum, Hordeum vulgare, and Zea mays by infecting an intact germinating seed with an agrobacterium containing a desired recombinant gene. ;SOLUTION: This is a method for more efficiently and more rapidly transforming a monocot such as Orya sative by removing a hull from a seed of the monocot, sterilizing the resultant intact seed in a solution such as 2.5% sodium hyposhlorite solution, adequately washing the sterilized seed with water, inoculating the washed seed onto a medium, incubating the inoculated seed at 27-32°C for 4-5 days for germination, soaking the incubated seed in a suspension of a transformed agrobacterium containing a desired recombinant gene to infect the seed with the transformed agrobacterium, transplanting the infected seed onto a medium, incubating the transplanted seed in the dark at 28°C for 3 days for co-culture, followed by washing the agrobacterium from the seed with a medium containing a herbicide such as carbenicillin after the co-culture.;COPYRIGHT: (C)2001,JPO