SEQUENTIAL ANALYTIC METHOD FOR GENE APPEARANCE

摘要

PROBLEM TO BE SOLVED: To provide a method capable of being matched with a nucleotide sequence in a database and/or selecting a cDNA clone in a library by using a short nucleotide sequence from a limited position in an mRNA or cDNA. SOLUTION: In the method for identifying the first nucleotide sequence with the second nucleotide sequence, the first nucleotide sequence having the limited position in a messenger RNA is selected in advance, the first nucleotide sequence is compared with the database of nucleotide sequences, the second nucleotide sequence in this database coincident with the first nucleotide sequence is selected and it is confirmed the second nucleotide sequence exists at the limited position in that messenger RNA. Thus, the method is provided with respective steps for identifying the first nucleotide sequence with the second nucleotide sequence, which shares the limited position and a common nucleotide sequence.