Biossensor for phenol based on blocking the transfer of electrons directly from peroxidase immobilized on su00eclica titanium

摘要

b "biossensor for phenol based on blocking the direct transfer of electrons of peroxidase immobilized on su00eclica titanium" d , the present invention relates to the immobilization of peroxidase on SiLica gel modified with titanium oxide and its use in the development of a biossensor amperomu00e9trico for phenol, the basis of the carbon paste.The transfer of electrons directly the reduction of hydrogen peroxide by the peroxidase activity was blocked when immobilized on silica, titanium,Obtain a biossensor with improved characteristics in terms of sensitivity, selectivity and operating range for the detection of phenol.This biossensor presented a very sensitive response to phenol in a potential applied 0 V versus ECs.The best conditions were achieved in phosphate buffer solution, pH 6.8, the ratio between the concentration of hydrogen peroxide / phenol greater than 0.35.Under these conditions, the biossensor showed a range of linear response between 10 and 50 u00c1mol L ^ - 1 ^ of phenol, adjusted by the equation (J = - 32.8 + 16.3 [phenol]For n = 5 with a correlation coefficient of 09995. The response time of the biossensor was about 3 s.We describe the possibility of selective determination of phenolic compounds based on differences in sensitivities recorded with this biossensor.When DNA was added together with the peroxidase enzyme in the stage of immobilization on the silica titanium, both the stability of the carbon paste modified with this material.Thrust into the refrigerator, such as the operational stability of the biossensor increased considerably.