DATA ANALYSIS AND DISPLAY SYSTEM FOR LIGATION-BASED DNA SEQUENCING

摘要

A data analysis system for ligation-based sequencing is provided. The systemincludes a base calling algorithm that may be implemented with a program ofinstructions (e.g., software) for determining a signature of a nucleotidesequence. A graphical user interface (GUI) enables a user to interact with andcontrol various aspects of the base calling algorithm. The base callingalgorithm and associated software may be used in connection with a techniquethat combines non-gel-based signature sequencing with in vitro cloning ofmillions of templates on separate 5 µm diameter microbeads.