Expression of the OSA gene in transgenic plants confers resistance to agrobacterium transformation and crown gall disease

摘要

When co-resident with the Ti-plasmid of iAgrobacterium tumefaciens/i, the iosa/i gene of the plasmid pSa causes oncogenic suppression of crown gall tumorigenesis by inhibiting transport of VirE2 protein from iAgrobacterium/i to the plant cell. To determine whether transgenic plants expressing iosa/i also are resistant to iAgrobacterium/i-mediated transformation, a Cauliflower Mosaic Virus (CaMV) 35S promoter-iosa/i-intron chimaeric gene was introduced into iArabidopsis thaliana/i and the resulting T2 and T3 progeny were tested for susceptibility to iAgrobacterium/i transformation, using as assays crown gall tumorigenesis, transformation to phosphinothricin-resistance, and the transient and stable expression of GUS activity. Of the 13 transgenic lines tested, 8 showed some level of resistance to subsequent iAgrobacterium/i transformation; several lines were highly resistant. Resistance to transformation co-segregated with the T-DNA containing the iosa/i transgene. This invention provides commercial use in protecting plants from crown gall disease by transforming them with the iosa/i gene.