Tapered bore column for high performance liquid chromatography

摘要

A column 20 is provided for use in high performance liquid chromatography systems. The column 20 has a bore 50 passing therethrough with an inlet 52 larger than an outlet 54. The side wall 56 of the bore 50 tapers as it extends between the inlet 52 and the outlet 54. The tapering bore 50 is packed with a chromatographic separation medium held within the tapering bore 50 by an inlet frit 38 adjacent an inlet 52 and an outlet frit 48 adjacent the outlet 54. The tapered bore column 20 can be substituted for standard cylindrical bore columns within high performance liquid chromatography systems to increase sample component resolution per unit time.