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FINGERPRINTING BACTERIAL STRAINS USING REPETITIVE DNA SEQUENCE AMPLIFICATION

机译:使用重复性DNA序列扩增技术对细菌菌株进行指印

摘要

we describe methods for oligonucleotide primers and identification of bacterial strains for determination of genomic fingerprints. these methods apply to a wide variety of samples. the test procedure involves the amplification of bacterial dna present in the sample to be tested, because in the latter two primers directed to the outside.they may hybridize with repetitive bacterial dna and extend to the outside from a repetitive sequence hybridable to another. after amplification, the products are sorted by size and by extension is determined by measuring the strain specific configuration of the products.the procedure for identifying bacterial strains by determining the fingerprints found many uses including: the identification of bacteria in infections, for agricultural or horticultural land, biomu00e9dication, food control, surveillan the production and assurance as well as quality control.
机译:我们描述了寡核苷酸引物和鉴定细菌菌株以确定基因组指纹的方法。这些方法适用于各种样品。测试程序涉及扩增待测样品中存在的细菌dna,因为在后两个引物中都指向外部,它们可能与重复的细菌dna杂交并从与另一个杂交的重复序列延伸至外部。扩增后,按大小分类产品,并通过测量产品的菌株特定构型确定延伸范围。通过确定发现的指纹来鉴定细菌菌株的程序具有许多用途,包括:鉴定感染的细菌,用于农业或园艺土地,生物制剂,食品控制,监视生产和保证以及质量控制。

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