HOMOGENEOUS BIOSPECIFIC ASSAY USING A SOLID PHASE, TWO-PHOTON EXCITATION AND CONFOCAL FLUORESCENCE DETECTION

摘要

It is an object of the present invention to the solution or biological suspensions of the analyte in a kind of uniform biospecific measuring method, wherein biospecific reagents competition ground binding analysis object and coordination are marked using fluorescent molecule, for with be bound to solid phase, and wherein there is being excited for the fluorophor two-photon excitation laser beam focus to arrive suitable for by two-photon excitation entering sample volume; The ligand of concentration and label detects the mark by photon transmitting contribution.