Solid phase, biospecific homogeneous assay using two-photon excitation and confocal fluorescence detection

摘要

(57) Abstract It is the method purpose of this invention concerning analysis things such as in the solution or in biological suspensoid being organism unique uniform assay method, reacting the organism unique reagent which competitively connects the ligand and aforementioned analysis ones which the sign are done with the solid phase with the fluorescent molecule, locking in the solid phase, using two photon excitation by making the laser beam focus which is suited for two photon excitation in sample liquid capacity, making the fluorescence of the ligand excite, detecting the density of the sign ligand through the photon discharge which is given and by the aforementioned sign.