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Time-resolved optical sensor array and ccd - cameras for frequency range - $fluorimetrically and / or phospholipids $rime
Time-resolved optical sensor array and ccd - cameras for frequency range - $fluorimetrically and / or phospholipids $rime
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机译:时间分辨光学传感器阵列和CCD-频率范围内的照相机-荧光计和/或磷脂计$ rime
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摘要
An apparatus for cross-correlation frequency domain fluorometry- phosphorimetry comprises a source of electromatic radiation and means for amplitude modulating the radiation at the first frequency. The amplitude modulated radiation is directed at a sample, while an optical array detector measures the resulting luminescence of the sample. A signal is provided coherent with the amplitude modulated electromagnetic radiation signals, at a second frequency which is different from the first frequency. The apparatus has the capability for shutting off and turning on the coherent signal at the second frequency in a cycle which is at a third frequency that is different from the difference between the first and second frequencies. This produces a resultant signal at a frequency derived from the difference and the third frequency. The resultant signal, when turned on, modulates the gain of the detecting means or multiplies its output, depending upon the nature of the detecting means. The amount of luminescence measured by the optical array detector is read when the coherent signal is off in its cycle of the third frequency. A signal from the reader described above is then detected at a frequency of the resultant signal to determine phase shift and modulation changes of the luminescence.
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