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Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody
Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody
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机译:可卡因衍生物,其蛋白质缀合物,产生单克隆抗体的细胞系,该细胞系的制备方法和单克隆抗体
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摘要
A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described. The method of the invention comprises preparing a cocaine-protein conjugate with a pyridyl dithiococaine derivative of Formula (I): ;where R is either H or R′; and where R′ is: ;wherein n=1 to 4 and Ph is a benzene ring, and wherein said monoclonal antibody detects 10−9 M cocaine; and which derivative binds to a protein by a disulfide bond; immunizing A/J mouse with the conjugate; fusing its spleen cell and a myeloma cell line; cloning the fusion to produce a monoclonal antibody producing cell line; culturing the cell line, and purifying a supernatant of the culture to form a monoclonal antibody to specifically bind to cocaine or cocaine derivatives.
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