Cocaine derivative, protein conjugate thereof, monoclonal antibody producing hybridoma cell line, method for preparing the hybridoma cell line and monoclonal antibody

摘要

A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described. The method of the invention comprises preparing a cocaine-protein conjugate with a pyridyl dithiococaine derivative of Formula (1): CHEM which binds to a protein by a disulfide bond, immunizing A/J mouse with the conjugate, fusing its spleen cell and a myeloma cell line, cloning the fusion to produce a monoclonal antibody producing cell line, culturing the cell line, and purifying a supernatant of the culture to form a monoclonal antibody to specifically bind to cocaine or cocaine derivatives.