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Methods for rapid cloning for full length cDNAs using a pooling strategy
Methods for rapid cloning for full length cDNAs using a pooling strategy
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机译:使用合并策略快速克隆全长cDNA的方法
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摘要
The present invention provides a method for high throughput cloning of full length cDNA sequences using a pooling strategy. This method uses a plurality of clone arrays prepared from cDNA libraries which have been preferably enriched for 5 ′ mRNA sequences and size fractionated into several discrete ranges (sub-libraries). These arrays are used to rapidly identify the full length cDNA sequence for a DNA segment of interest.
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