METHODS FOR RAPID CLONING OF FULL LENGTH CDNAS

摘要

The present invention provides a method for high throughput cloning of fulllength cDNA sequences. This method uses a plurality of clone arrays preparedfrom cDNA libraries which have been preferably enriched for 5' mRNA sequencesand size fractionated into several discrete ranges (sub-libraries). Thesearrays are used to rapidly identify the full length cDNA sequence for a DNAsegment of interest.