Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells

摘要

It is formed the present invention relates to neuron and the method for the treatment of disease, it is characterised in that abnormal dopaminergic activity (DA) and contain thrombocytin (5HT) neuron. Particularly, the present invention relates to one kind forms the external neuroprogenitor cells of serotonergic neuron and a effective amount of native sequence by contact, variant and function fragment FGF-4, FGF-8 with hiss. A kind of dopaminergic neuron neuroprogenitor cells and a effective amount of FGF-8 are formed by contact and hissed in addition, disclosing. What is further described is composition, cell culture compositions and medical device, these compositions contain enough FGF-8, is hissed or FGF-8, hisses and is divided into dopaminergic or serotonergic neuron is respectively connected with to stimulate with FGF-4. What is further described is application method) serotoneurgic neurons treat the related food intake of disorder, hormone secretion, stress reaction, pain and immune function, sexual behaviour decline, cardiovascular function and temperature are adjusted, it is especially depressed, it is inclined to and takes one's own life by means of a sword, brute force attack behavior, anancastic behavior and anorexia/bulimia and schizophrenia. What is further described is application method) dopaminergic neuron treats the relevant attitudinal reflex of disorder, move and reward relevant behavior, specifically, Parkinson& apos; S diseases, schizophrenia and drug habit. It further describes, applies a kind of neuronal survival factor, for example, NGF, CNTF, BDNF, NT-3, NT-4, aFGF, IL-1& bgr; TNF&agr; IGF-1, IGF-2, TGF-& bgr; TGF-&bgr; 1 or Skeletal Muscle Extract.