Production of rearrangement monerin which uses methylotroph yeast revelation system

摘要

(57) Abstract This invention, the single chain monerin way regards the protein. This single chain monerin way the protein is stability, 100 times it is sweet at least when it compares with the sucrose and on the basis of weight. In addition as for this invention, this monerin way it regards the nuclear acid which the protein the cord/code is done. It is desirable, this nuclear acid furthermore in methylotroph yeast Pichia pastoris, in order the cord/code to be done monerin, includes the promoter and signal arrangement in order to point the revelation and the secretion of the protein. This invention furthermore, this monerin way the rearrangement Pichia pastoris cell which includes the nuclear acid which the protein the cord/code is done, from this rearrangement Pichia pastoris the monerin way regards the product of process, and this process in order to produce the protein.