Rice plant white leaf blight resistant gene Xa1 and Xa1 protein

摘要

PROBLEM TO BE SOLVED: To obtain the subject new protein having a specific amino acid sequence, resistant to Xanthomonas oryzae pv. oryzae, and useful for breeding gene-recombined rice resistant to the Xanthomonas oryzae pv. oryzae by the transduction of the gene. SOLUTION: This new Xa-1 protein comprises an amino acid sequence of the formula and resists to Xanthomonas oryzae pv. oryzae. The transduction of the gene resistant to rice Xanthomonas oryzae pv. oryzae is useful for breeding the gene-recombined rice exhibiting the resistance to the rice Xanthomonas oryzae pv. oryzae. The gene is obtained by inoculating Xanthomonas oryzae pv. oryzae I group in the mature leaves of a strain resistant to the rice Xanthomonas oryzae pv. oryzae, extracting mRNA from the inoculated leaves within 5 days, preparing a cDNA library from the mRNA, isolating and identifying resistant gene candidates from the cDNA library by the use of a probe, narrowing the obtained candidates, inserting the obtained Xa-1 into an expressing vector, and subsequently expressing the gene Xa-1 in a host cell.