Methods for identifying a mismatch repair glycosylase reactive sites, compounds and use thereof

摘要

(57) [Abstract] The present application discloses a method of identifying a mutation in the target DNA sequence. Including the steps of the method: the method comprising hybridizing a control DNA sequence and sequence DNA targets (a), wherein the control DNA sequence is a wild type DNA sequence, corresponding to the target DNA sequence to form a duplex Te, step: by treatment (b) of the duplex, also remove aldehyde of any naturally-generating step; (c) can be reacted with repair glycosylase the duplex, any mismatch site of the double-chain, it is converted to a reactive site comprising an aldehyde-containing abasic sites, step: for a time and under conditions sufficient for covalent attachment to the (d) reactive sites, the method comprising reacting a compound of the formula X-Z-Y of the duplex, where, X is a detectable moiety, Y is Z NHNH _2, O-NH _2, or and is NH ₂ is an alkyl amine hydrocarbons, alkyl hydroxy, alkyl ethoxy, alkyl esters, alkyl ethers, alkyl or amide, and Z, Z is cut and may be unsubstituted or may be substituted To determine the mismatch or cause a (f) where the step; may include the groups, the process; by detecting a compound which is (e) binding, to identify the site of the mismatch, the step (g) the mismatch and There determines whether a polymorphism or a mutation process.