首页>
外国专利>
Computer program product for quantitative analysis of a nucleic acid amplification reaction
Computer program product for quantitative analysis of a nucleic acid amplification reaction
展开▼
机译:用于定量分析核酸扩增反应的计算机程序产品
展开▼
页面导航
摘要
著录项
相似文献
摘要
A computer program product for determining a threshold value (e.g., a threshold cycle number or time value) in a nucleic acid amplification reaction tangibly embodies instructions readable by a machine to perform the steps of deriving a growth curve from measurements of a signal whose intensity is related to a quantity of nucleic acid sequence being amplified in the reaction, calculating a derivative of the growth curve, identifying a characteristic of the derivative, and determining a threshold value associated with the characteristic of the derivative. The method provides for highly reproducible threshold values that are independent of noise or background signal in the amplification reaction. Embodiments of a computer program product for determining a starting quantity of a nucleic acid sequence in a test sample are also provided.
展开▼