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Methods to overexpress a foreign gene in a cell or in an animal in vitro and in vivo
Methods to overexpress a foreign gene in a cell or in an animal in vitro and in vivo
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机译:在体外和体内在细胞或动物中过表达外源基因的方法
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摘要
The present invention provides a nucleic acid molecule comprising: (a) a region of DNA which is homologous to a region of an endogenous gene present in a genome of a cell of interest; (b) a first nucleic acid encoding an encephalomyocarditis internal ribosome entry site (EMCV IRES); (c) a second nucleic acid encoding a selectable marker which can be excised from the nucleic acid molecule if the nucleic acid molecule has been integrated into the genome of the cell of interest; and (d) a third nucleic acid encoding a gene of interest. The cell may be an animal cell, a yeast cell or a plant cell. The invention also provides for transgenic non-human animals which are created using the above described construct. The invention also provides methods for making such transgenic animals.
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