Compound fluorescent marker, assay method using said composite marker and kit for testing

摘要

"Compound fluorescent marker, assay method using said composite marker and kit for testing".The present invention relates to a pigment cianina fluorscente of general formula: in which: x1, X2 are independently selected from the group consisting of - O -, - S -,- C (CH ~ 3 ~) ~ 2 ~ or - C = ch ~ 2 ~; ~ y ~ 1.Y ~ 2 ~ are atoms of non metals required to form a condensed ring or naphtho - benzo condensed; q is a half combined which increases the flurescu00eancia and stability of the compound; R ~ 1 ~ R ~ 2 ~ are i.Ndependentemente selected among the group consisting of H, alkyl groups C ~ 1 ~ C ~ 4 ~.Alquilenosulfu00f3nicos groups or groups alquilenosulfonato in which the alkylene group has 1 to 4 carbon atoms; R ~ 3 ~.R ~ 4 ~ R ~ 5 ~ are independently selected from the group consisting of H, a sulfonic group, a group of sulfonate groups alquilenosulfu00f3nico,Alquilenosulfonato and only ~ 2 ~ NH (CH ~ 2 ~) ~ m ~ - W - (CH ~ 2 ~) ~ n ~ Z, in which the alkylene has 1 to 4 carbon atoms.On condition that at least one of R ~ 1 ~ R ~ 5 ~ contains a sulphonic acid or sulfonate group; W is absent or is a group selected from among only ~ 2 ~ NH -, - O -, - COO - or - you know -; n is 0 to 12.With the conditions of M + n 243 12 and at least one of M and N is different from 0, and Z is, or contains, a feature nucleofu00edlica n, O or s,Or is or contains a functionality capable of reacting with nucleophiles n, O or S.The features nucleofu00edlicas include NH ~ 2 ~, - Oh, and - SH groups capable of reacting with; such features include - COCI, coocor, conhnh ~ 2 ~ n - hidroxisuccinimido esters, NCS, Cho.- choch ~ 2 ~ I, fosforamidita and maleimido.