Methods of producing a panto compound, pantothenate and beta alanine, increasing the production of a panto compound and edentificar compounds that modulate the activity of pantothenate kinase.The recombinant vector, microorganism, acid molecule nuleico isolated, and proteu00ecna pantothenate kinase isolated from polipetu00ecdeo

摘要

Methods of producing a panto compound, pantothenate and beta alanine, increasing the production of a panto compound and to identify compounds that modulate the activity of pantothenate kinase.The recombinant vector, microorganism, molecule of nucleic acid isolation, polipetu00ecdeo proteu00ecna pantothenate kinase and the isolated. The present invention describes methods of producing panto compounds (for example,Pantothenate) using microorganisms in the path biossintu00e9tu00edco of pantothenate biosynthetic and / or the way of isoleucine, valine and / or biosynthetic path of coenzimaa has been manipulated.The methods of microorganisms that supraexpressam the cetopantoato reductase as well as microorganisms that supraexpressam aspartate decarboxylase - 244 is provided.Methods of producing panto compounds in a way independent of the precursor and high yield are described.Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products are useful in the practice of the methods above are also provided.The present invention also portrays a microbial pantothenate kinase gene not identified previously, coax,As well as methods to produce panto compounds using microorganisms having the modified pantothenate kinase activity.Recombinant microorganisms, vectors, nucleic acid molecules isolated and purified coax coax proteins are depicted.They also reveal methods to identify modulators of pantothenate kinase using recombinant microorganisms and / or the protein of the present invention purified coax.