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PREPARATION FOR CELLULAR SYNTHESIS OF PROTEIN, CONTAINING CELLULAR EXTRACTS, AND METHOD OF ITS APPLICATION FOR SYNTHESIS OF PROTEINS

机译:包含细胞提取物的蛋白质的细胞合成的制备方法及其在蛋白质合成中的应用方法

摘要

Disclosed are: a preparation containing cellular extracts for cell-free protein synthesis, obtained by removing the system inhibiting the synthesis of endogenous protein; a device for carrying out cell-free protein synthesis, equipped with a chamber for the reaction of cell-free protein synthesis; and a set designed for this; the drug can be stored at room temperature with preservation of the biological functions of cellular extracts; In addition, a method for cell-free protein synthesis is disclosed using cellular extracts from which inhibitors of the reaction of synthesis of endogenous proteins are removed, in which components such as mRNA serving as a synthesis reaction matrix, an enzyme of the energy regeneration system, a substrate and an energy source can be added, stored replace or delete. The international application was published along with an international search report.
机译:公开了一种通过去除抑制内源蛋白质合成的系统而获得的,用于无细胞蛋白质合成的含有细胞提取物的制剂。用于进行无细胞蛋白质合成的装置,其具有用于进行无细胞蛋白质合成的反应的室。和为此设计的一套;该药物可以在室温下保存,并保留细胞提取物的生物学功能。另外,公开了一种使用细胞提取物的无细胞蛋白质合成方法,该细胞提取物去除了内源蛋白质合成反应的抑制剂,其中诸如mRNA等成分用作合成反应基质,能量再生系统的酶。 ,可以添加,存储替换或删除基板和能源。国际申请与国际检索报告一同发布。

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