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Protein, processes for hydrolyzing beta-glucosidic and beta-xylosidic bonds and for producing a polypeptide, nucleic acid sequence, nucleic acid construct, recombinant microbial strain, and oligonucleotide probe
Protein, processes for hydrolyzing beta-glucosidic and beta-xylosidic bonds and for producing a polypeptide, nucleic acid sequence, nucleic acid construct, recombinant microbial strain, and oligonucleotide probe
The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins,. and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.
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机译:本发明涉及对应于家族7和10的金孢菌糖基水解酶的新蛋白,其与SEQ ID No 2和4的氨基酸序列分别表现出最小氨基酸同一性为70和75%。金孢属甘油醛磷酸酯脱氢酶,与SEQ ID No.6的部分氨基酸序列具有至少86%的氨基酸同一性。本发明还涉及编码这些蛋白质的核酸序列。尤其是调控相应基因表达的启动子序列。表达这些基因的优选宿主是真菌,特别是金孢菌属菌株。
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