Transformation and regeneration of genetically modified plants and plant materials from Eucalyptus and Pinus species

摘要

Methods are described for producing genetically modified plants, particularly woody plants, and most particularly plants of the Eucalyptus and Pinus species. The methods involve transformation of target plant material with a desired genetic construct and regeneration of the transformed plant material using an adventitious shoot bud system. The methods provide high transformation efficiency and reduce the duration of the transformation and regeneration protocols. Stem segments of a target plant are transformed using Agrobacterium-mediated techniques, and adventitious shoot buds are regenerated from the Agrobacterium-infected stem segments. Preferred culture media, including selection media, and improved plant culture techniques are disclosed.