Method for identifying ligands specific for progesterone receptor isoforms of progesterone Receptors and ligands Selective for Fabrics

摘要

It relates to a method for identifying ligands specific for the isoform of the progesterone receptor (PR) or b) that includes: to stably transfect cells as a Group 1 CellsHuman neuroblastoma with a Plasmid as promoter of the mouse mammary tumour virus that expresses the receptor isoform PR to stably transfect; (b) a group of cells with a P 2Lasmido expresses the receptor isoform PR B; c) transfecting groups 1 and 2 cells with a Plasmid that contains a Gene informant as the Luciferase Gene.Connected with a promoter that responds to hormones; (d) put in Contact Group 1 and 2 cells with a ligand to evaluate; (e) to determine the efficacy of gene Transcription or power sourceIn groups 1 and 2 cells; (f) to select a ligand that has a selectivity for receptor isoform PR a or B; the difference in the efficiency of Transcription is greater or equal to 10%.The difference of the power of 10That is determined by Measuring the activity of the Luciferase reporter gene Plasmid; and also comprise a Gene of Resistance to antibiotics such as Neomycin or puromycin.It also relates to a method for identifying receptor ligands Selective for tissues that also includes PR (g) to submit the selected ligand in vivo tests in a First tissue (mLoves) and a Second tissue (uterus) White, (H) select the ligand that has the desired activity