HUMAN INTERFERON-.BETA.2A AND INTERFERON-.BETA.2B; VECTORS CONTAINING GENES CODING FOR SAID INTERFERONS: CELL LINES PRODUCING SAME AND USE OF SAID INTERFERONS AS PHARMACEUTICALS

摘要

Human interferon-beta2A and interferon-beta2B are produced in purifiedform by recombinant DNA techniques. Two separate human genes havebeen identified which code for the production of IFN-.beta.2A and IFN-.beta.2B,respectively. The sequence of IFN-.beta.2A cDNA is established. Thesegenes and cDNA have been cloned into mammlian cells with an SV40early promoter sequence and such genomic clones are capable of pro-ducing IFN-.beta.2A and IFN-.beta.2B. The antaviral activity of suchrecombinant IFN-.beta.2A and IFN-.beta.2B is demonstrated as well as otherbiological activity identifying them as human interferons. It has beenshown that IFN-.beta.2 secretion is induced in human cells by growth-stimulatory cytokines.