METHOD FOR IN VIVO, EX VIVO AND IN VITRO REPAIR AND REGENERATION OF CARTILAGE AND COLLAGEN AND BONE REMODELING

摘要

A method for in vivo, ex vivo and in vitro regeneration of cartilage andcollagen. in vivo, ex vivo and in vitro regeneration and de novo formation ofarticular cartilage and collagen by intermittently applied hydrostaticpressure. The application of external interval loading consisting of repeatedperiods of applied hydrostatic pressure followed and interrupted by periods ofrecovery. The application of the intermittent hydrostatic pressure atphysiological levels 5-10 MPa for an interval of 4 hours followed by arecovery period up to about 20 hours, said pressure applied to the cartilagecells in vitro, explants of cartilage ex vivo and in vivo to cartilage thatremains intact within the joint space of diarthrotic joints. The intervalloading results in the selective inhibition of matrix degrading enzymes, pro-inflammatory cytokines and chemokines that attract inflammatory cells into thejoint cavity and in selective decrease of gene expression of growth factorsthat are inhibitory to type II collagen expression.