Assay for cell cycle modulators based on the modulation of cyclin d1 degradationin response to ionising radiation

摘要

The present invention relates to the finding that cyclin D1 is targeted for destruction in cells which have been exposed to ionising radiation (IR). This finding gives rise to an assay for modulators of cell cycle control, which assay comprises: (a) providing a cell in culture together with a potential modulator compound, said cell expressing a cyclin D1 which undergoes degradation in response to DNA damage; (b) exposing said cell to a DNA damaging agent; and (c) determining the extent to which the presence of the potential modulator compound inhibits the degradation of said cyclin D1. This finding further gives rise to an assay for modulators of cell cycle control, which assay comprises: (a) providing a cyclin D1, the APC or a compound thereof which interacts with cyclin D1, together with a potential modulator compound; and (b) determining the extent to which the presence of the potential modulator compound inhibits the interaction of said cyclin D1 and APC or component thereof. In particular where the component of the APC is a protein cdc20.