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Geranyl diphosphate synthase from mint (mentha piperita)

机译:薄荷中的香叶基二磷酸合酶(mentha piperita)

摘要

A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.
机译:已经分离并测序了编码来自薄荷的香叶基二磷酸合酶大亚基的cDNA,并确定了相应的氨基酸序列。提供了可复制的重组克隆载体,其编码香叶基二磷酸合酶大亚基。在另一方面,提供了修饰的宿主细胞,其已经用重组克隆载体和/或编码香叶基二磷酸合酶大亚基的DNA序列转化,转染,感染和/或注射。在另一方面,本发明提供了分离的,重组的香叶基二磷酸合酶蛋白,其包含分离的,重组的香叶基二磷酸合酶大亚基蛋白和分离的重组的香叶基二磷酸合酶小亚基蛋白。因此,提供了用于重组表达香叶基二磷酸合酶的系统和方法。

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