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RECOMBINANT AVIDIN MONOMER AND ITS USE IN BIOTIN BINDING
RECOMBINANT AVIDIN MONOMER AND ITS USE IN BIOTIN BINDING
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机译:重组亲和素单体及其在生物素结合中的应用
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摘要
Chicken avidin, a homotetramer that binds four molecules of biotin was converted to a monomeric form by successive mutations of interface residues to alanine. The major contribution to monomer formation was the mutation of two aspartic acid residues, which together account for ten hydrogen bonding interactions at the 1-4 interface. Mutation of these residues, together with the three hydrophobic residues at the 1-3 interface, led to stable monomer formation in the absence of biotin. Upon addition of biotin, the monomeric avidin reassociated to the tetramer, which exhibited properties similar to those of native avidin, with respect to biotin binding, thermostability, and protease resistance.
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