HUMAN SOURCE GENE LEADING SEQUENCE, GENE VECTOR AND GENE EXPRESSION STRATEGY

摘要

The invention disclosed a human source leading sequence, a gene vector and a gene expression method. It involves (1) the DNA sequence in short arms of human D, G group chromosome, which does not represent any important physiological function, and the homologue DNA sequence. Such sequence is used as the leading sequence of target gene; (2) Constructing a gene vector which comprises the above leading sequence; (3) Introducing target gene into the nucleolus region in D, G group chromosome of human cell mentioned in step (1), where the expression is active. This invention provides a novel gene leading sequence, a gene vector which is constructed by using this sequence and a method for the gene expression. The vector can be applied to human gene therapy and producing the protein of interests.