HUMAN SOURCE GENE LEADING SEQUENCE, GENE VECTOR AND GENE EXPRESSION STRATEGY

摘要

The p resent i nvention d eals w ith the leading sequence o f human source gene, gene vector and gene expression strategies. The invention include the following: (1) Using DNA sequence without pivot physiological function-related gene in short arms of human group D, G chromosomes, or a DNA sequence sharing 50% or over 50% identity to the sequence select from human D, G group chromosomes as leading sequence for gene targeting; (2) Construct the gene vector containing the leading sequence above; (3) Nucleolus organizing region in D, G group chromosomes mentioned in the procedure(1) is used as the target site, the gene of interest is integrated into the short arms where the gene expresses actively in D,G group chromosomes of human cells. The present invention provides a novel gene leading sequence by which the gene vector construction and gene expression strategies are obtained. The gene expression strategies can be used for human gene therapy and manufacturing protein.